Review



anti cd70 antibody  (Bio X Cell)


Bioz Verified Symbol Bio X Cell is a verified supplier
Bioz Manufacturer Symbol Bio X Cell manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Bio X Cell anti cd70 antibody
    (A-B) Graphs show XCR1 (A) and SIRPa (B) signal density within innate-infiltrated and non-infiltrated regions on stained spleen sections harvested 24hrs after LM-OVA infection (n=5). C- Mice were adoptively transferred with OTIxGFP T-cells and infected with LM-OVA. After 24hrs, spleen sections were stained for CD169 (red), 33D1 (magenta), XCR1 (cyan), OTI (green). Representative image. (D-F) Mice were infected with LM-OVA for 24hrs and splenocytes were analysed by flow cytometry. Graphs show CD80 MFI ( D , n=5-7), CD86 MFI ( E , n=5-7), and frequency of <t>CD70+</t> cDC1 and cDC2 ( F , n=4-5). (G-H) Spleen sections from mice infected with LM-OVA for 24hrs were stained for CD169 (blue), CD70 (red), CD11b (white). G- Representative image. H- Graph shows CD70 signal intensity within innate-infiltrated and non-infiltrated regions in WPs (n=5). I-K Mice were infected with LM-OVA and treated with anti-CXCR3 or anti-CD70 antibodies as indicated 6hrs before infection. Graphs show frequencies (I) , absolute numbers (J) and avidity (K) of N4-tetramer+ CD8 T-cells 8 days post-infection (n=12-17 per group). Ratio paired t-tests (A-B, D-F, H) , Welch and Brown-Forsythe one-way ANOVA with Dunnett’s multiple comparisons test (I-K) .
    Anti Cd70 Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd70+antibody/InVivoMAb+anti-mouse+CD70/bio_rxiv__64898__2026__03__18__712682-230-8-10
    Average 93 stars, based on 36 article reviews
    anti cd70 antibody - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Transient immune landscape remodelling shapes CD8 T-cell priming during infection"

    Article Title: Transient immune landscape remodelling shapes CD8 T-cell priming during infection

    Journal: bioRxiv

    doi: 10.64898/2026.03.18.712682

    (A-B) Graphs show XCR1 (A) and SIRPa (B) signal density within innate-infiltrated and non-infiltrated regions on stained spleen sections harvested 24hrs after LM-OVA infection (n=5). C- Mice were adoptively transferred with OTIxGFP T-cells and infected with LM-OVA. After 24hrs, spleen sections were stained for CD169 (red), 33D1 (magenta), XCR1 (cyan), OTI (green). Representative image. (D-F) Mice were infected with LM-OVA for 24hrs and splenocytes were analysed by flow cytometry. Graphs show CD80 MFI ( D , n=5-7), CD86 MFI ( E , n=5-7), and frequency of CD70+ cDC1 and cDC2 ( F , n=4-5). (G-H) Spleen sections from mice infected with LM-OVA for 24hrs were stained for CD169 (blue), CD70 (red), CD11b (white). G- Representative image. H- Graph shows CD70 signal intensity within innate-infiltrated and non-infiltrated regions in WPs (n=5). I-K Mice were infected with LM-OVA and treated with anti-CXCR3 or anti-CD70 antibodies as indicated 6hrs before infection. Graphs show frequencies (I) , absolute numbers (J) and avidity (K) of N4-tetramer+ CD8 T-cells 8 days post-infection (n=12-17 per group). Ratio paired t-tests (A-B, D-F, H) , Welch and Brown-Forsythe one-way ANOVA with Dunnett’s multiple comparisons test (I-K) .
    Figure Legend Snippet: (A-B) Graphs show XCR1 (A) and SIRPa (B) signal density within innate-infiltrated and non-infiltrated regions on stained spleen sections harvested 24hrs after LM-OVA infection (n=5). C- Mice were adoptively transferred with OTIxGFP T-cells and infected with LM-OVA. After 24hrs, spleen sections were stained for CD169 (red), 33D1 (magenta), XCR1 (cyan), OTI (green). Representative image. (D-F) Mice were infected with LM-OVA for 24hrs and splenocytes were analysed by flow cytometry. Graphs show CD80 MFI ( D , n=5-7), CD86 MFI ( E , n=5-7), and frequency of CD70+ cDC1 and cDC2 ( F , n=4-5). (G-H) Spleen sections from mice infected with LM-OVA for 24hrs were stained for CD169 (blue), CD70 (red), CD11b (white). G- Representative image. H- Graph shows CD70 signal intensity within innate-infiltrated and non-infiltrated regions in WPs (n=5). I-K Mice were infected with LM-OVA and treated with anti-CXCR3 or anti-CD70 antibodies as indicated 6hrs before infection. Graphs show frequencies (I) , absolute numbers (J) and avidity (K) of N4-tetramer+ CD8 T-cells 8 days post-infection (n=12-17 per group). Ratio paired t-tests (A-B, D-F, H) , Welch and Brown-Forsythe one-way ANOVA with Dunnett’s multiple comparisons test (I-K) .

    Techniques Used: Staining, Infection, Flow Cytometry

    (A-B) Representative image of spleen sections from mice infected with LM-OVA for 24hrs were stained for CD169 (blue), XCR1 (cyan), NKp46 (red) and SIRPa ( A , magenta), or 33D1 ( B , magenta). C- Mice were adoptively transferred with OTIxGFP cells and infected with LM-OVA. Representative image of spleen sections stained for CD169 (red), 33D1 (magenta), XCR1 (cyan), OTI (green) 24hrs post-infection. (D-E) Splenocytes from mice infected with LM-OVA for 24hrs were analysed by flow cytometry. D- Representative gating strategy for splenic cDC1 and cDC2 subsets. E- Representative histograms showing CD80, CD86, and CD70 expression in cDC1 and cDC2.
    Figure Legend Snippet: (A-B) Representative image of spleen sections from mice infected with LM-OVA for 24hrs were stained for CD169 (blue), XCR1 (cyan), NKp46 (red) and SIRPa ( A , magenta), or 33D1 ( B , magenta). C- Mice were adoptively transferred with OTIxGFP cells and infected with LM-OVA. Representative image of spleen sections stained for CD169 (red), 33D1 (magenta), XCR1 (cyan), OTI (green) 24hrs post-infection. (D-E) Splenocytes from mice infected with LM-OVA for 24hrs were analysed by flow cytometry. D- Representative gating strategy for splenic cDC1 and cDC2 subsets. E- Representative histograms showing CD80, CD86, and CD70 expression in cDC1 and cDC2.

    Techniques Used: Infection, Staining, Flow Cytometry, Expressing

    Related Articles

    Control:

    Article Title: Transient immune landscape remodelling shapes CD8 T-cell priming during infection
    Article Snippet: For CXCR3 blockade, mice were intraperitoneally administered with 300μg anti-CXCR3 (BioLegend, 126537) or isotype control antibody (BioLegend, 400959) 6hr before infection, unless otherwise stated. .. For CD70 blockade, mice were intraperitoneally administered 300μg anti-CD70 antibody (BioXCell, BE0022) or isotype control antibody (BioXcell, BE0290) 6hr before infection, either alone or in combination with CXCR3 blockade. .. For Treg depletion experiments, FoxP3-DTR mice were intraperitoneally administered 500ng diphtheria toxin (Merck, 322326) on two consecutive days.

    Infection:

    Article Title: Transient immune landscape remodelling shapes CD8 T-cell priming during infection
    Article Snippet: For CXCR3 blockade, mice were intraperitoneally administered with 300μg anti-CXCR3 (BioLegend, 126537) or isotype control antibody (BioLegend, 400959) 6hr before infection, unless otherwise stated. .. For CD70 blockade, mice were intraperitoneally administered 300μg anti-CD70 antibody (BioXCell, BE0022) or isotype control antibody (BioXcell, BE0290) 6hr before infection, either alone or in combination with CXCR3 blockade. .. For Treg depletion experiments, FoxP3-DTR mice were intraperitoneally administered 500ng diphtheria toxin (Merck, 322326) on two consecutive days.

    Injection:

    Article Title: CD 70 encoded by modified vaccinia virus Ankara enhances CD 8 T‐cell‐dependent protective immunity in MHC class II ‐deficient mice
    Article Snippet: .. Where noted, anti‐CD70 antibody (clone FR70, Bio X Cell) was injected i.v. at the indicated doses and time‐points. ..



    Similar Products

    anti cd70 pe  (Miltenyi Biotec)
    93
    Miltenyi Biotec anti cd70 pe
    Anti Cd70 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd70+antibody/CD70+Antibody%2C+anti-mouse/us12559535-248-94-100
    Average 93 stars, based on 1 article reviews
    anti cd70 pe - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    anti cd70 antibody  (Bio X Cell)
    93
    Bio X Cell anti cd70 antibody
    (A-B) Graphs show XCR1 (A) and SIRPa (B) signal density within innate-infiltrated and non-infiltrated regions on stained spleen sections harvested 24hrs after LM-OVA infection (n=5). C- Mice were adoptively transferred with OTIxGFP T-cells and infected with LM-OVA. After 24hrs, spleen sections were stained for CD169 (red), 33D1 (magenta), XCR1 (cyan), OTI (green). Representative image. (D-F) Mice were infected with LM-OVA for 24hrs and splenocytes were analysed by flow cytometry. Graphs show CD80 MFI ( D , n=5-7), CD86 MFI ( E , n=5-7), and frequency of <t>CD70+</t> cDC1 and cDC2 ( F , n=4-5). (G-H) Spleen sections from mice infected with LM-OVA for 24hrs were stained for CD169 (blue), CD70 (red), CD11b (white). G- Representative image. H- Graph shows CD70 signal intensity within innate-infiltrated and non-infiltrated regions in WPs (n=5). I-K Mice were infected with LM-OVA and treated with anti-CXCR3 or anti-CD70 antibodies as indicated 6hrs before infection. Graphs show frequencies (I) , absolute numbers (J) and avidity (K) of N4-tetramer+ CD8 T-cells 8 days post-infection (n=12-17 per group). Ratio paired t-tests (A-B, D-F, H) , Welch and Brown-Forsythe one-way ANOVA with Dunnett’s multiple comparisons test (I-K) .
    Anti Cd70 Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd70+antibody/InVivoMAb+anti-mouse+CD70/bio_rxiv__64898__2026__03__18__712682-230-8-10
    Average 93 stars, based on 1 article reviews
    anti cd70 antibody - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    medium  (Miltenyi Biotec)
    93
    Miltenyi Biotec medium
    (A-B) Graphs show XCR1 (A) and SIRPa (B) signal density within innate-infiltrated and non-infiltrated regions on stained spleen sections harvested 24hrs after LM-OVA infection (n=5). C- Mice were adoptively transferred with OTIxGFP T-cells and infected with LM-OVA. After 24hrs, spleen sections were stained for CD169 (red), 33D1 (magenta), XCR1 (cyan), OTI (green). Representative image. (D-F) Mice were infected with LM-OVA for 24hrs and splenocytes were analysed by flow cytometry. Graphs show CD80 MFI ( D , n=5-7), CD86 MFI ( E , n=5-7), and frequency of <t>CD70+</t> cDC1 and cDC2 ( F , n=4-5). (G-H) Spleen sections from mice infected with LM-OVA for 24hrs were stained for CD169 (blue), CD70 (red), CD11b (white). G- Representative image. H- Graph shows CD70 signal intensity within innate-infiltrated and non-infiltrated regions in WPs (n=5). I-K Mice were infected with LM-OVA and treated with anti-CXCR3 or anti-CD70 antibodies as indicated 6hrs before infection. Graphs show frequencies (I) , absolute numbers (J) and avidity (K) of N4-tetramer+ CD8 T-cells 8 days post-infection (n=12-17 per group). Ratio paired t-tests (A-B, D-F, H) , Welch and Brown-Forsythe one-way ANOVA with Dunnett’s multiple comparisons test (I-K) .
    Medium, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd70+antibody/CD70+Antibody%2C+anti-human%2C+REAfinity/10__3390_slash_biomedicines14010045-51-40-42
    Average 93 stars, based on 1 article reviews
    medium - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    stemmacs ips brew xf medium  (Miltenyi Biotec)
    93
    Miltenyi Biotec stemmacs ips brew xf medium
    (A-B) Graphs show XCR1 (A) and SIRPa (B) signal density within innate-infiltrated and non-infiltrated regions on stained spleen sections harvested 24hrs after LM-OVA infection (n=5). C- Mice were adoptively transferred with OTIxGFP T-cells and infected with LM-OVA. After 24hrs, spleen sections were stained for CD169 (red), 33D1 (magenta), XCR1 (cyan), OTI (green). Representative image. (D-F) Mice were infected with LM-OVA for 24hrs and splenocytes were analysed by flow cytometry. Graphs show CD80 MFI ( D , n=5-7), CD86 MFI ( E , n=5-7), and frequency of <t>CD70+</t> cDC1 and cDC2 ( F , n=4-5). (G-H) Spleen sections from mice infected with LM-OVA for 24hrs were stained for CD169 (blue), CD70 (red), CD11b (white). G- Representative image. H- Graph shows CD70 signal intensity within innate-infiltrated and non-infiltrated regions in WPs (n=5). I-K Mice were infected with LM-OVA and treated with anti-CXCR3 or anti-CD70 antibodies as indicated 6hrs before infection. Graphs show frequencies (I) , absolute numbers (J) and avidity (K) of N4-tetramer+ CD8 T-cells 8 days post-infection (n=12-17 per group). Ratio paired t-tests (A-B, D-F, H) , Welch and Brown-Forsythe one-way ANOVA with Dunnett’s multiple comparisons test (I-K) .
    Stemmacs Ips Brew Xf Medium, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd70+antibody/CD70+Antibody%2C+anti-human%2C+REAfinity/10__3390_slash_biomedicines14010045-51-37-42
    Average 93 stars, based on 1 article reviews
    stemmacs ips brew xf medium - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    xf medium  (Miltenyi Biotec)
    93
    Miltenyi Biotec xf medium
    (A-B) Graphs show XCR1 (A) and SIRPa (B) signal density within innate-infiltrated and non-infiltrated regions on stained spleen sections harvested 24hrs after LM-OVA infection (n=5). C- Mice were adoptively transferred with OTIxGFP T-cells and infected with LM-OVA. After 24hrs, spleen sections were stained for CD169 (red), 33D1 (magenta), XCR1 (cyan), OTI (green). Representative image. (D-F) Mice were infected with LM-OVA for 24hrs and splenocytes were analysed by flow cytometry. Graphs show CD80 MFI ( D , n=5-7), CD86 MFI ( E , n=5-7), and frequency of <t>CD70+</t> cDC1 and cDC2 ( F , n=4-5). (G-H) Spleen sections from mice infected with LM-OVA for 24hrs were stained for CD169 (blue), CD70 (red), CD11b (white). G- Representative image. H- Graph shows CD70 signal intensity within innate-infiltrated and non-infiltrated regions in WPs (n=5). I-K Mice were infected with LM-OVA and treated with anti-CXCR3 or anti-CD70 antibodies as indicated 6hrs before infection. Graphs show frequencies (I) , absolute numbers (J) and avidity (K) of N4-tetramer+ CD8 T-cells 8 days post-infection (n=12-17 per group). Ratio paired t-tests (A-B, D-F, H) , Welch and Brown-Forsythe one-way ANOVA with Dunnett’s multiple comparisons test (I-K) .
    Xf Medium, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd70+antibody/CD70+Antibody%2C+anti-human%2C+REAfinity/pmc12837672-37-40-43
    Average 93 stars, based on 1 article reviews
    xf medium - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    anti cd70  (Cell Signaling Technology Inc)
    93
    Cell Signaling Technology Inc anti cd70
    (A-B) Graphs show XCR1 (A) and SIRPa (B) signal density within innate-infiltrated and non-infiltrated regions on stained spleen sections harvested 24hrs after LM-OVA infection (n=5). C- Mice were adoptively transferred with OTIxGFP T-cells and infected with LM-OVA. After 24hrs, spleen sections were stained for CD169 (red), 33D1 (magenta), XCR1 (cyan), OTI (green). Representative image. (D-F) Mice were infected with LM-OVA for 24hrs and splenocytes were analysed by flow cytometry. Graphs show CD80 MFI ( D , n=5-7), CD86 MFI ( E , n=5-7), and frequency of <t>CD70+</t> cDC1 and cDC2 ( F , n=4-5). (G-H) Spleen sections from mice infected with LM-OVA for 24hrs were stained for CD169 (blue), CD70 (red), CD11b (white). G- Representative image. H- Graph shows CD70 signal intensity within innate-infiltrated and non-infiltrated regions in WPs (n=5). I-K Mice were infected with LM-OVA and treated with anti-CXCR3 or anti-CD70 antibodies as indicated 6hrs before infection. Graphs show frequencies (I) , absolute numbers (J) and avidity (K) of N4-tetramer+ CD8 T-cells 8 days post-infection (n=12-17 per group). Ratio paired t-tests (A-B, D-F, H) , Welch and Brown-Forsythe one-way ANOVA with Dunnett’s multiple comparisons test (I-K) .
    Anti Cd70, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd70+antibody/CD70+Antibody/pm41344992-46-18-22
    Average 93 stars, based on 1 article reviews
    anti cd70 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    anti human cd70 apc vio 770  (Miltenyi Biotec)
    93
    Miltenyi Biotec anti human cd70 apc vio 770
    (A-B) Graphs show XCR1 (A) and SIRPa (B) signal density within innate-infiltrated and non-infiltrated regions on stained spleen sections harvested 24hrs after LM-OVA infection (n=5). C- Mice were adoptively transferred with OTIxGFP T-cells and infected with LM-OVA. After 24hrs, spleen sections were stained for CD169 (red), 33D1 (magenta), XCR1 (cyan), OTI (green). Representative image. (D-F) Mice were infected with LM-OVA for 24hrs and splenocytes were analysed by flow cytometry. Graphs show CD80 MFI ( D , n=5-7), CD86 MFI ( E , n=5-7), and frequency of <t>CD70+</t> cDC1 and cDC2 ( F , n=4-5). (G-H) Spleen sections from mice infected with LM-OVA for 24hrs were stained for CD169 (blue), CD70 (red), CD11b (white). G- Representative image. H- Graph shows CD70 signal intensity within innate-infiltrated and non-infiltrated regions in WPs (n=5). I-K Mice were infected with LM-OVA and treated with anti-CXCR3 or anti-CD70 antibodies as indicated 6hrs before infection. Graphs show frequencies (I) , absolute numbers (J) and avidity (K) of N4-tetramer+ CD8 T-cells 8 days post-infection (n=12-17 per group). Ratio paired t-tests (A-B, D-F, H) , Welch and Brown-Forsythe one-way ANOVA with Dunnett’s multiple comparisons test (I-K) .
    Anti Human Cd70 Apc Vio 770, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd70+antibody/CD70+Antibody%2C+anti-human%2C+REAfinity/pmc12645426-114-116-119
    Average 93 stars, based on 1 article reviews
    anti human cd70 apc vio 770 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    fr70  (Bio X Cell)
    93
    Bio X Cell fr70
    (A-B) Graphs show XCR1 (A) and SIRPa (B) signal density within innate-infiltrated and non-infiltrated regions on stained spleen sections harvested 24hrs after LM-OVA infection (n=5). C- Mice were adoptively transferred with OTIxGFP T-cells and infected with LM-OVA. After 24hrs, spleen sections were stained for CD169 (red), 33D1 (magenta), XCR1 (cyan), OTI (green). Representative image. (D-F) Mice were infected with LM-OVA for 24hrs and splenocytes were analysed by flow cytometry. Graphs show CD80 MFI ( D , n=5-7), CD86 MFI ( E , n=5-7), and frequency of <t>CD70+</t> cDC1 and cDC2 ( F , n=4-5). (G-H) Spleen sections from mice infected with LM-OVA for 24hrs were stained for CD169 (blue), CD70 (red), CD11b (white). G- Representative image. H- Graph shows CD70 signal intensity within innate-infiltrated and non-infiltrated regions in WPs (n=5). I-K Mice were infected with LM-OVA and treated with anti-CXCR3 or anti-CD70 antibodies as indicated 6hrs before infection. Graphs show frequencies (I) , absolute numbers (J) and avidity (K) of N4-tetramer+ CD8 T-cells 8 days post-infection (n=12-17 per group). Ratio paired t-tests (A-B, D-F, H) , Welch and Brown-Forsythe one-way ANOVA with Dunnett’s multiple comparisons test (I-K) .
    Fr70, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd70+antibody/InVivoMAb+anti-mouse+CD70/us12473377-216-78-80
    Average 93 stars, based on 1 article reviews
    fr70 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    (A-B) Graphs show XCR1 (A) and SIRPa (B) signal density within innate-infiltrated and non-infiltrated regions on stained spleen sections harvested 24hrs after LM-OVA infection (n=5). C- Mice were adoptively transferred with OTIxGFP T-cells and infected with LM-OVA. After 24hrs, spleen sections were stained for CD169 (red), 33D1 (magenta), XCR1 (cyan), OTI (green). Representative image. (D-F) Mice were infected with LM-OVA for 24hrs and splenocytes were analysed by flow cytometry. Graphs show CD80 MFI ( D , n=5-7), CD86 MFI ( E , n=5-7), and frequency of CD70+ cDC1 and cDC2 ( F , n=4-5). (G-H) Spleen sections from mice infected with LM-OVA for 24hrs were stained for CD169 (blue), CD70 (red), CD11b (white). G- Representative image. H- Graph shows CD70 signal intensity within innate-infiltrated and non-infiltrated regions in WPs (n=5). I-K Mice were infected with LM-OVA and treated with anti-CXCR3 or anti-CD70 antibodies as indicated 6hrs before infection. Graphs show frequencies (I) , absolute numbers (J) and avidity (K) of N4-tetramer+ CD8 T-cells 8 days post-infection (n=12-17 per group). Ratio paired t-tests (A-B, D-F, H) , Welch and Brown-Forsythe one-way ANOVA with Dunnett’s multiple comparisons test (I-K) .

    Journal: bioRxiv

    Article Title: Transient immune landscape remodelling shapes CD8 T-cell priming during infection

    doi: 10.64898/2026.03.18.712682

    Figure Lengend Snippet: (A-B) Graphs show XCR1 (A) and SIRPa (B) signal density within innate-infiltrated and non-infiltrated regions on stained spleen sections harvested 24hrs after LM-OVA infection (n=5). C- Mice were adoptively transferred with OTIxGFP T-cells and infected with LM-OVA. After 24hrs, spleen sections were stained for CD169 (red), 33D1 (magenta), XCR1 (cyan), OTI (green). Representative image. (D-F) Mice were infected with LM-OVA for 24hrs and splenocytes were analysed by flow cytometry. Graphs show CD80 MFI ( D , n=5-7), CD86 MFI ( E , n=5-7), and frequency of CD70+ cDC1 and cDC2 ( F , n=4-5). (G-H) Spleen sections from mice infected with LM-OVA for 24hrs were stained for CD169 (blue), CD70 (red), CD11b (white). G- Representative image. H- Graph shows CD70 signal intensity within innate-infiltrated and non-infiltrated regions in WPs (n=5). I-K Mice were infected with LM-OVA and treated with anti-CXCR3 or anti-CD70 antibodies as indicated 6hrs before infection. Graphs show frequencies (I) , absolute numbers (J) and avidity (K) of N4-tetramer+ CD8 T-cells 8 days post-infection (n=12-17 per group). Ratio paired t-tests (A-B, D-F, H) , Welch and Brown-Forsythe one-way ANOVA with Dunnett’s multiple comparisons test (I-K) .

    Article Snippet: For CD70 blockade, mice were intraperitoneally administered 300μg anti-CD70 antibody (BioXCell, BE0022) or isotype control antibody (BioXcell, BE0290) 6hr before infection, either alone or in combination with CXCR3 blockade.

    Techniques: Staining, Infection, Flow Cytometry

    (A-B) Representative image of spleen sections from mice infected with LM-OVA for 24hrs were stained for CD169 (blue), XCR1 (cyan), NKp46 (red) and SIRPa ( A , magenta), or 33D1 ( B , magenta). C- Mice were adoptively transferred with OTIxGFP cells and infected with LM-OVA. Representative image of spleen sections stained for CD169 (red), 33D1 (magenta), XCR1 (cyan), OTI (green) 24hrs post-infection. (D-E) Splenocytes from mice infected with LM-OVA for 24hrs were analysed by flow cytometry. D- Representative gating strategy for splenic cDC1 and cDC2 subsets. E- Representative histograms showing CD80, CD86, and CD70 expression in cDC1 and cDC2.

    Journal: bioRxiv

    Article Title: Transient immune landscape remodelling shapes CD8 T-cell priming during infection

    doi: 10.64898/2026.03.18.712682

    Figure Lengend Snippet: (A-B) Representative image of spleen sections from mice infected with LM-OVA for 24hrs were stained for CD169 (blue), XCR1 (cyan), NKp46 (red) and SIRPa ( A , magenta), or 33D1 ( B , magenta). C- Mice were adoptively transferred with OTIxGFP cells and infected with LM-OVA. Representative image of spleen sections stained for CD169 (red), 33D1 (magenta), XCR1 (cyan), OTI (green) 24hrs post-infection. (D-E) Splenocytes from mice infected with LM-OVA for 24hrs were analysed by flow cytometry. D- Representative gating strategy for splenic cDC1 and cDC2 subsets. E- Representative histograms showing CD80, CD86, and CD70 expression in cDC1 and cDC2.

    Article Snippet: For CD70 blockade, mice were intraperitoneally administered 300μg anti-CD70 antibody (BioXCell, BE0022) or isotype control antibody (BioXcell, BE0290) 6hr before infection, either alone or in combination with CXCR3 blockade.

    Techniques: Infection, Staining, Flow Cytometry, Expressing